| dc.contributor.author | Wintrip, Norman. | en_US |
| dc.date.accessioned | 2014-10-21T12:37:31Z | |
| dc.date.available | 1996 | |
| dc.date.issued | 1996 | en_US |
| dc.identifier.other | AAINN15834 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10222/55111 | |
| dc.description | Immunocytochemical detection of Fos protein was used to test the hypothesis that c-fos expression in the PVN is regulated by neural pathways which are modulated by adrenal and gonadal steroids. | en_US |
| dc.description | Basal levels of Fos-like immunoreactivity (FLI) were very low in the PVN but rose following adrenalectomy (ADX), unilateral ADX (uADX) or sham ADX (30 minutes-8 hours; ADX $\ge$ sham ADX $>$ uADX). No circadian variation in basal or ADX-induced FLI was observed. Opposite effects were seen in two supposedly hyporesponsive models, i.e., Lewis rats displayed smaller responses to uADX or sham ADX compared to Sprague-Dawley rats, but adrenal surgery (AS) induced more FLI in 3 and 11 day old rats compared to adults. FLI response to AS was attenuated by exogenous corticosterone, but FLI levels were potentiated after similar surgeries (ovariectomy or laparotomy) in previously (7 days) ADX rats. Lactating females (LF; postpartum day 7-21) showed a profound suckling-dependent inhibition of the FLI response to AS. This attenuation was also noted in LFs treated with NMA (a glutamate agonist) or Metrazole (a convulsant). | en_US |
| dc.description | The influence of sex steroids on c-fos gene expression in the PVN was revealed using the NMDA antagonist MK801. MK801 (1 mg/kg) induced FLI in the PVN of males, females, and lactating females (M $>$ F $>$ LF). At lower doses, (0.1 or 0.3 mg./kg.) sex differences were reversed (F $>$ M). Effects of gonadectomy and androgenization were inconclusive but suggest that observed sex differences might be androgen dependent. At all doses tested, behavioural anomalies were greater in females. | en_US |
| dc.description | These results emphasize the difficulty of isolating the specific effects of experimental manipulations from those of non-specific stressors. Data presented suggest that quantification of FLI in the PVN is a useful approach to study the influence of adrenal and gonadal steroids on PVN function, and that the lactating female rat and Lewis rat should be useful models for further investigations in this area. | en_US |
| dc.description | Thesis (Ph.D.)--Dalhousie University (Canada), 1996. | en_US |
| dc.language | eng | en_US |
| dc.publisher | Dalhousie University | en_US |
| dc.publisher | | en_US |
| dc.subject | Biology, Neuroscience. | en_US |
| dc.subject | Biology, Animal Physiology. | en_US |
| dc.title | The regulation ofc-fos induction in the hypothalamic paraventricular nucleus. | en_US |
| dc.type | text | en_US |
| dc.contributor.degree | Ph.D. | en_US |
