Show simple item record

dc.contributor.authorSidik, Saima
dc.date.accessioned2013-06-18T17:05:32Z
dc.date.available2013-06-18T17:05:32Z
dc.date.issued2013-06-18
dc.identifier.urihttp://hdl.handle.net/10222/22282
dc.description.abstractShigellae are pathogenic bacteria that cause the disease shigellosis. Two methods for studying secreted effectors encoded by this pathogen’s virulence plasmid are described. First, protein microarrays were used to identify substrates of an E3 ubiquitin ligase called IpaH7.8. Second, a deletion collection containing mutants for every gene on the virulence plasmid was used in two screens: one to identify mutants that elicit atypical levels of Interleukin-8 (IL-8) from U937 cells, and one to identify mutants that bind the dye Congo red abnormally. Although protein microarrays were an ineffective tool, the deletion collection proved valuable. Most mutants were less effective at sequestering Congo red than wild-type S. flexneri, although this ability was enhanced in several mutants. Four mutants, ?ospB, ?orf186, ?mxiH and ?mxiK, elicited higher levels of IL-8 from U937 cells than wild type S. flexneri. These results validate the use of the deletion collection as a tool for studying bacterial pathogenesis.en_US
dc.language.isoenen_US
dc.subjectShigellaen_US
dc.subjectbacteriologyen_US
dc.subjectType III Secretionen_US
dc.subjectGenetic Toolsen_US
dc.subjectUbiquitinen_US
dc.subjectIpaHsen_US
dc.titleTOOLS FOR IDENTIFYING FUNCTIONS OF TYPE III SECRETION SYSTEM EFFECTORS FROM SHIGELLA FLEXNERIen_US
dc.date.defence2012-04-17
dc.contributor.departmentDepartment of Microbiology & Immunologyen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinerDr. Song Leeen_US
dc.contributor.graduate-coordinatorDr. Roy Duncanen_US
dc.contributor.thesis-readerDr. Brent Johnstonen_US
dc.contributor.thesis-supervisorDr. John Rohde, Dr. Jean Marshallen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseNot Applicableen_US
 Find Full text

Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record