Electrospray Ionization Tandem-MS of Ammonium and Alkali Metals of TAGs and their Oxidation Products with Fractionation by LC-MS and FAIMS
Abstract
Triacylglycerols (TAGs) are the main component of edible oils, where unsaturated TAGs are easily oxidized, leading to toxicity and negative health effects. The analysis of TAG and TAG oxidation products is an ongoing analytical challenge due to the immense complexity within the molecules. Previous studies on TAG oxidation focused on hydroperoxide products to measure oxidation. There are few studies that address the importance of hydroxide and epoxide formation and their effect on health and nutritional deterioration. In this thesis, a longitudinal liquid chromatography- tandem mass spectrometry (LC-MSMS) study was presented for the simultaneous qualification and quantification of TAGs and their oxidation products in canola oil. The method allowed the oil sample to be diluted 100,000-fold, while avoiding other sample preparation steps and reducing variability in results.