Evaluation of Lysozyme Product (InovapureTM) as a Sanitizer for Broiler Hatching Eggs

Abstract

Hatching egg sanitation is a practice to achieve successful production in the commercial hatchery. Trials were conducted to investigate the effectiveness of EDTA modified lysozyme against E. coli penetrating eggshells and its effects on hatching performance and growth performance. In trial one, floor-collected eggs were fumigated with five levels of lysozyme for two exposure times. In trial two, cage-collected eggs were inoculated with E. coli then sanitized. In trial one, 2.25% and 3.00% lysozyme solution reduced the penetration of E. coli into eggs without affecting hatching performance and growth performance. In trial two, 3.00% lysozyme demonstrated activity against E. coli on eggshells, and provided continuous bactericidal action to prevent E. coli penetration. The application of lysozyme decreased the presence of E. coli in yolk sac of newly hatched chicks. 1.50% lysozyme solution improved hatch weight without affecting growth performance. Lysozyme can be an effective and safe sanitizer for hatching eggs.