| dc.contributor.author | Franck, Jens P. C. | en_US |
| dc.date.accessioned | 2014-10-21T12:37:46Z | |
| dc.date.available | 1993 | |
| dc.date.issued | 1993 | en_US |
| dc.identifier.other | AAINN87454 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10222/55356 | |
| dc.description | I have cloned and sequenced members of two satellite DNA families, SATA and SATB, from the genomes of the tilapiine and haplochromine fishes. The SATA satellite DNAs were visualized as intensely staining bands after electrophoretic separation of EcoRI-digested (tilapiine species) and HinfI-digested (H. similis) genomic DNA. Monomer repeats were cloned from tilapiine fishes of the three major genera: Oreochromis, Sarotherodon, and Tilapia as well as the haplochromine species Haplochromis similis. Three size-variants of approximately 237 bp (type I), 230 bp (type II), and 209 bp (type III) were identified. Pairwise comparison of derived consensus sequences for the SATA monomers revealed identities ranging from 74 to 97%. The type II and type III size-variants appear to have arisen by deletions of 9 and 29 bp, respectively, within different regions of the type I satellite. Molecular phylogenetic analyses of the tilapiine SATA sequences support the close relationship of the two mouthbrooding genera, Oreochromis and Sarotherodon, with a clear dichotomy between this clade and the substrate spawning Tilapia genus. | en_US |
| dc.description | The SATB satellite DNA is a 1900 bp EcoRI fragment cloned from the genome of the tilapiine fish Oreochromis niloticus. A 760 bp HindIII fragment, an internal component of SATB, was cloned and sequenced from the related tilapiine species, Oreochromis hornorum. The SATB repeat is more widely conserved than the SATA repeat and was detected in the genomic DNA of African and neotropical cichlid species. Four imperfect 21 bp direct repeat sequences were present within the cloned 1900 bp EcoRI repeat. Alignment of the four direct repeats revealed a core motif of 11 bp that exhibits 100% sequence identity between all of the direct repeats. The conservation of this motif in the SATB repeat suggests that it is under selective constraint. | en_US |
| dc.description | Thesis (Ph.D.)--Dalhousie University (Canada), 1993. | en_US |
| dc.language | eng | en_US |
| dc.publisher | Dalhousie University | en_US |
| dc.publisher | | en_US |
| dc.subject | Biology, Molecular. | en_US |
| dc.title | Organization and evolution of two satellite DNA families, SATA and SATB, from the tilapiine and haplochromine genome (Pisces: Cichlidae). | en_US |
| dc.type | text | en_US |
| dc.contributor.degree | Ph.D. | en_US |
