| dc.contributor.author | Moore, Celine. | en_US |
| dc.date.accessioned | 2014-10-21T12:38:53Z | |
| dc.date.available | 1991 | |
| dc.date.issued | 1991 | en_US |
| dc.identifier.other | AAINN76729 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10222/55306 | |
| dc.description | The project was aimed at studying the effect(s) of interleukin-1 (IL-1) and testicular macrophages on androgen release by adult rat Leydig cells in vitro in the context of the paracrine regulation of Leydig cell function. Purified Leydig cells were cultured with or without IL-1$\alpha$, usually for 72 h, followed by 4 h in the presence of LH, dbcAMP or pregnenolone. Arachidonic acid metabolism inhibitors were also used. The medium was collected daily and assayed for androgen and, in some cases, cAMP and/or progesterone content. | en_US |
| dc.description | IL-1$\alpha$ increased basal androgen release and enhanced the effect of submaximally effective concentrations of LH (less than 1 ng/ml), dbcAMP (30 $\mu$M) and pregnenolone (0.3 $\mu$M). IL-1$\alpha$ thus seems to stimulate steroido-genesis at a step distal to pregnenolone formation. IL-1$\alpha$ increased basal cAMP release but this effect occured later than the increase in androgen. Blocking the lipoxygenase pathway of arachidonic acid metabolism with NDGA resulted in a gradual but complete disappearance of this effect. Cyclooxygenase inhibitors were without effect. | en_US |
| dc.description | IL-1$\alpha$ had a dose- and time-dependent inhibitory effect on the response to 100 ng LH/ml. IL-1$\alpha$ also inhibited the response to 30 $\mu$M dbcAMP and 3 $\mu$M pregnenolone indicating that IL-1$\alpha$ has an inhibitory effect on the 17$\alpha$-hydroxylase/C$\sb{17-20}$ lyase. LH-induced cAMP levels were also inhibited after 3 days with IL-1$\alpha$ suggesting the existence of more than one site of action. Lipoxygenase and cyclooxygenase inhibitors did not affect the inhibitory effect of IL-1$\alpha$. Testicular macrophage conditioned medium (TMCM) had a stimulatory effect on both basal and LH-stimulated androgen release, suggesting that IL-1 is not the active factor in the TMCM. | en_US |
| dc.description | Taken together the data suggest that IL-1$\alpha$ stimulates androgen release but also decreases the maximum steroidogenic capability of the cells. The data also support the hypothesis that an IL-1-like molecule and testicular macrophages are involved in the paracrine regulation of Leydig cell steroidogenesis. | en_US |
| dc.description | Thesis (Ph.D.)--Dalhousie University (Canada), 1991. | en_US |
| dc.language | eng | en_US |
| dc.publisher | Dalhousie University | en_US |
| dc.publisher | | en_US |
| dc.subject | Biology, Cell. | en_US |
| dc.subject | Biology, Animal Physiology. | en_US |
| dc.title | The effect of interleukin-1alpha and testicular macrophages on adult rat Leydig cell steroidogenesis in vitro. | en_US |
| dc.type | text | en_US |
| dc.contributor.degree | Ph.D. | en_US |
