| dc.contributor.author | Zhang, Min. | en_US |
| dc.date.accessioned | 2014-10-21T12:34:53Z | |
| dc.date.available | 1990 | |
| dc.date.issued | 1990 | en_US |
| dc.identifier.other | AAINN64517 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10222/55205 | |
| dc.description | Electrical impedance was measured in carrot root, potato tuber, and red onion scale tissues using a multi-electrode system to eliminate electrode impedance. The tissue impedance was analyzed according to three major equivalent linear electrical model circuits: (i) an R-C parallel circuit; (ii) a single shell model including extracellular resistance, symplasmic resistance and membrane capacitance, (iii) a double shell model including extracellular resistance, cytoplasmic resistance, vacuole interior resistance, plasma membrane capacitance and tonoplast capacitance. When each model was fitted to the complex impedance spectrum, using complex nonlinear least squares (CNLS), it was found that in all cases the double shell model best represented the plant tissues tested. | en_US |
| dc.description | Application of this technique of impedance analysis to a few plant tissues under freeze-thaw stress conditions clearly indicated that electrolyte leakage from apoplast to symplast is a major symptom of non-lethal freeze-thaw injury. Such non-lethal freeze-thaw injury is immediately detectable upon thawing, by a continuous record of low frequency AC impedance. Based on the findings that in situ electrolyte leakage occurs mainly during a brief period associated with thawing and that non-lethal freeze-thaw injury has a similar pattern of leakage to that of lethal injury, it is proposed that membrane micro-rupture and subsequent repealing during thawing could be responsible for the significant electrolyte leakage occurring in non-lethal injury in plant tissues in general. Compared with the real-time impedance recording, previous estimations of membrane permeability in freeze-thaw stressed tissue using the tissue leaching method may not be reliable because the rate of leaching is influenced by both the rate of leakage from symplasm to apoplasm, and the rate of leakage from apoplasm to the bathing solution. | en_US |
| dc.description | Thesis (Ph.D.)--Dalhousie University (Canada), 1990. | en_US |
| dc.language | eng | en_US |
| dc.publisher | Dalhousie University | en_US |
| dc.publisher | | en_US |
| dc.subject | Agriculture, Plant Culture. | en_US |
| dc.title | Electrical impedance analysis and freeze-thaw injury in plants. | en_US |
| dc.type | text | en_US |
| dc.contributor.degree | Ph.D. | en_US |
