| dc.contributor.author | Kaur, Bhavleen. | en_US |
| dc.date.accessioned | 2014-10-21T12:37:34Z | |
| dc.date.available | 2004 | |
| dc.date.issued | 2004 | en_US |
| dc.identifier.other | AAINQ93284 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10222/54633 | |
| dc.description | The morphology, ultrastructure, biochemical characteristics and 16S rRNA gene sequence of a bacterium, designated strain BH1, isolated from the rusticles that were removed from the wreck of the Royal Mail Steamship (RMS) Titanic, were determined. Strain BH1 was rod-shaped, Gram-negative and produced circular, off-white, opaque colonies on marine agar. Both long and short rod forms, ranging in size from 2--6 mum in length and 0.5--0.8 mum in width, were observed. Transmission electron microscopy revealed that strain BH1 had a typical Gram-negative cell wall structure and possessed 2--6 peritrichous flagella. 16S rRNA gene sequence comparison placed strain BH1 in the genus Halomonas of the family Halomonadaceae within class gammaproteobacteria of the phylum Proteobacteria. It showed 98% sequence similarity with the species Halomonas variabilis. However, cells of H. variabilis are vibrio-shaped, monoflagellated and obligately aerobic. This implies that strain BH1 could be a novel species within the family. | en_US |
| dc.description | In addition, the biocorrosive ability of the strain BH1 was studied by testing its effect on corrosion of mild steel coupons. Strain BH1 cells were able to adhere to the metal surface, and caused the formation of tubercles. Localised corrosion was observed under these tubercles, implicating the role of strain BH1 in microbially influenced corrosion. ESEM and EDX analysis inferred the presence of goethite and green rust crystals within the corrosion products. Both these formations are abundantly present in rusticles. Thus, this indicates that strain BH1 may have been part of a transient consortium that was involved in the formation of the rusticles, on the Titanic. | en_US |
| dc.description | Thesis (Ph.D.)--Dalhousie University (Canada), 2004. | en_US |
| dc.language | eng | en_US |
| dc.publisher | Dalhousie University | en_US |
| dc.publisher | | en_US |
| dc.subject | Biology, Microbiology. | en_US |
| dc.subject | Biology, Oceanography. | en_US |
| dc.title | Investigation of the identity and biocorrosive ability of a novel deep-sea bacterium, belonging to the genus Halomonas, from the Titanic. | en_US |
| dc.type | text | en_US |
| dc.contributor.degree | Ph.D. | en_US |
