dc.contributor.author | Fernando, T R G Wasundara | |
dc.date.accessioned | 2014-08-25T16:03:07Z | |
dc.date.available | 2014-08-25T16:03:07Z | |
dc.date.issued | 2014-08-25 | |
dc.identifier.uri | http://hdl.handle.net/10222/54032 | |
dc.description.abstract | Phloridzin (PZ), a polyphenol compound found in apple, exerts glucose transporter inhibitory and estrogen-like effects. Therapeutic applications of PZ are limited due to its poor bioavailability. In this study, anitiproliferative efficacy of docosahexaenoic acid (DHA) acylated PZ (PZ-DHA) was investigated using a triple negative breast cancer cell line (MDA-MB-231). PZ-DHA, but not PZ, caused the death of MDA-MB-231 in vitro which was both time- and dose-dependent. PZ-DHA-induced apoptosis was indicated by DNA fragmentation and activation of caspase 3/7. However, reactive oxygen species production was not required for PZ-DHA-mediated cell death. PZ-DHA showed low cytotoxicity toward human mammary epithelial cells. Moreover, in vivo tumor suppresser effect of PZ-DHA was observed when MDA-MB-231 cells xenografted non-obese diabetic severe combined immune-deficient (NOD-SCID) mice were subjected to intratumoral injections of PZ-DHA. Anticancer efficacy observed in cell cultures and mice warrant further investigations of PZ-DHA as a potential treatment for breast cancer. | en_US |
dc.subject | Triple negative breast cancer, tumor suppression, NOD-SCID, phloridzin, docosahexaenoic acid, PZ-DHA | en_US |
dc.title | EFFICACY AND SAFETY OF DOCOSAHEXAENOIC ACID ACYLATED PHLORIDZIN FOR TREATING TRIPLE NEGATIVE BREAST CANCER | en_US |
dc.date.defence | 2014-08-13 | |
dc.contributor.department | Faculty of Agriculture | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | Dr. Paola Marcato | en_US |
dc.contributor.graduate-coordinator | Dr. Dian Patterson | en_US |
dc.contributor.thesis-reader | Dr. David W. Hoskin | en_US |
dc.contributor.thesis-reader | Dr. Bernhard Benkel | en_US |
dc.contributor.thesis-supervisor | Dr. H. P. Vasantha Rupasinghe | en_US |
dc.contributor.ethics-approval | Received | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |