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dc.contributor.authorAmpaw, Anna A.
dc.date.accessioned2018-02-12T17:13:17Z
dc.date.available2018-02-12T17:13:17Z
dc.identifier.urihttp://hdl.handle.net/10222/73603
dc.description.abstractβ-Phosphoglucomutase (βPGM) is an isomerase that catalyzes the conversion of β-glucose 1-phosphate to glucose 6-phosphate via a 1,6-bisphosphate intermediate. Incorporation of 5-fluorotryptophan (5FW) into βPGM (ie. 5FWβPGM) as a 19F NMR spectroscopic probe revealed that the 19F nucleus in 5FW is a sensitive probe for monitoring metal fluoride transition state analogue (TSA) complexation and ligand binding. The ability of βPGM to form transition state analogue complexes with novel compounds, β-glucose 1,6C-phosphonate, and β-2-deoxy-2-fluoro glucose and mannose 1C-phosphonates were examined; however, only β-glucose 1,6C-phosphonate (IC50 = 186 +/- 73 µM) and β-2-deoxy-2-fluoro glucose (2.68 +/- 0.04 µM) configured analogs bound to βPGM. All compounds that exhibited complexation with 5FWβPGM by 19F NMR spectroscopy were confirmed as competitive. Approaches to the synthesis of 1,6-bisphosphate and phosphofluoridates analogs as mechanism-based inactivators of 5FWβPGM are described.en_US
dc.language.isoenen_US
dc.subjectOrganic Chemistryen_US
dc.subjectEnzymologyen_US
dc.subjectEnzyme kineticsen_US
dc.subjectNuclear magnetic resonance (NMR)en_US
dc.titleThe Expression of 19F-Labeled Beta-Phosphoglucomutase and the Evaluation of Its Inhibitorsen_US
dc.date.defence2016-07-28
dc.contributor.departmentDepartment of Chemistryen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinern/aen_US
dc.contributor.graduate-coordinatorHeather A. Andreasen_US
dc.contributor.thesis-readerBruce Grindley, Stephen Bearneen_US
dc.contributor.thesis-supervisorDavid L. Jakemanen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNoen_US
dc.contributor.copyright-releaseNot Applicableen_US
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