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dc.contributor.authorTorabi zadeh, Maryam
dc.date.accessioned2017-04-13T11:53:38Z
dc.date.available2017-04-13T11:53:38Z
dc.identifier.urihttp://hdl.handle.net/10222/72847
dc.description.abstractWhey protein is a by-product of cheese manufacturing and a rich source of bioactive peptides. However, it is an important cause of food allergy in many patients that are allergic to bovine milk. Enzymatic hydrolysis is an approach that has been used for the production of hypoallergenic products. The impact of hydrolysis with various enzymes on immunochemical properties, hydrophobicity and particle size of whey protein isolate was studied. Twelve different enzymes were used of which papain and pancreatin hydrolysate showed the highest immunoglobulin (Ig) E inhibition of 47% and 45% among all the hydrolysates. A strong negative correlation between the degree of hydrolysis and hydrophobicity was observed. The peptidomics result of papain hydrolysate was analyzed and enzymatic cleavage sites were determined. Comparison of in silico and actual results indicated that antigenic epitopes were mostly degraded. This demonstrates antigenic inhibition in the papain whey hydrolysate obtained in vitro.en_US
dc.language.isoenen_US
dc.subjectWhey proteinen_US
dc.subjectEnzymatic hydrolysisen_US
dc.subjectCleavage specificityen_US
dc.subjectAntigenic epitopesen_US
dc.subjectSuppressing immunoreactivityen_US
dc.subjectPeptidomicsen_US
dc.subjectPapain hydrolysateen_US
dc.subjectWhey
dc.subjectWhey products
dc.titleAPPLICATION OF PROTEOLYTIC ENZYMES IN SUPPRESSING IMMUNOREACTIVITY AND DEACTIVATION OF ANTIGENIC EPITOPES IN THE WHEY PROTEIN ISOLATEen_US
dc.date.defence2017-03-07
dc.contributor.departmentFaculty of Agricultureen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinern/aen_US
dc.contributor.graduate-coordinatorDr. Haibo Niuen_US
dc.contributor.thesis-readerDr. Dian Pattersonen_US
dc.contributor.thesis-readerDr. Nancy Pittsen_US
dc.contributor.thesis-readerDr. Randal Olsonen_US
dc.contributor.thesis-supervisorDr. Chibuike C. Udenigween_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseNot Applicableen_US
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