| dc.contributor.author | WALL, MARK | |
| dc.contributor.author | D'CUNHA, GODWIN | |
| dc.date.accessioned | 2017-02-10T19:08:57Z | |
| dc.date.available | 2017-02-10T19:08:57Z | |
| dc.date.issued | 2006 | |
| dc.identifier.uri | http://hdl.handle.net/10222/72686 | |
| dc.description | 2005 NSIS Undergraduate Student Prize Winning Paper in the Life Sciences | en_US |
| dc.description.abstract | A procedure is described for the synthesis of L-phenylalanine from trans-cinnamic
acid and ammonia utilizing the reverse reaction of phenylalanine ammonia lyase (PAL)
from Rhodotoru/a glutinis. Optimal conditions determined for yeast growth and PAL
production were pH 6, 1% inoculum, 30°C for 26-28 h. Optimization of conditions for
maximal phenylalanine production included incubation time (16 -18 h), temperature
(30°C), pH (9 .0), selected concentrations of substrates: 0.1 M trans-cinnamic acid and
4.0M NH40H and 0.1M Tris-HCI. Under these conditions about 85% conversion of
substrate to product was obtained. | en_US |
| dc.language.iso | en_US | en_US |
| dc.publisher | Nova Scotian Institute of Science | en_US |
| dc.relation.ispartof | Proceedings of the Nova Scotian Institute of Science | en_US |
| dc.title | GROWTH AND PHENYLALANINE AMMONIA LYASE ACTIVITY OF Rhodotorula g/utinis: OPTIMIZATION OF CONDITIONS FOR THE SYNTHESIS OF L-PHENYLALANINE | en_US |
| dc.type | Article | en_US |
| dc.identifier.volume | 43 | |
| dc.identifier.issue | 2 | |
| dc.identifier.startpage | 219 | |
