Recent Submissions

  • Growth and the cell cycle of the yeast Saccharomyces cerevisiae. II. Relief of cell-cycle constraints allows accelerated cell divisions 

    Singer, R. A., and G. C. Johnston. 1983. "Growth and the cell cycle of the yeast Saccharomyces cerevisiae. II. Relief of cell-cycle constraints allows accelerated cell divisions." Experimental cell research 149(1): 15-26.
    For cells of the yeast Saccharomyces cerevisiae, conditions which limit S phase or nuclear division allow steady-state division kinetics without significant effects on growth. Such cells become unusually large. When large ...
  • A cyclin protein modulates mitosis in the budding yeast Saccharomyces cerevisiae 

    Veinot-Drebot, L. M., G. C. Johnston, and R. A. Singer. 1991. "A cyclin protein modulates mitosis in the budding yeast Saccharomyces cerevisiae." Current genetics 19(1): 15-19.
    For the budding yeast Saccharomyces cerevisiae the mitotic cell cycle is coordinated with cell mass at the regulatory step 'start'. The threshold amount of cell mass (reflected as a 'critical size') necessary for 'start' ...
  • Heat shock causes transient inhibition of yeast rRNA gene transcription 

    Veinot-Derbot, L. M., R. A. Singer, and G. C. Johnston. 1989. "Heat shock causes transient inhibition of yeast rRNA gene transcription." Journal of Biological Chemistry 264(33): 19473-19474.
    The heat-shock response in the yeast Saccharomyces cerevisiae includes transiently decreased production of the full-size pre-rRNA transcript. Here we have used quantitative hybridization of pulse-labeled RNA to cloned, ...
  • Mating ability during chemically induced G1 arrest of cells of the yeast Saccharomyces cerevisiae 

    Bedard, D. P., A. W. Li, R. A. Singer, and G. C. Johnston. 1984. "Mating ability during chemically induced G1 arrest of cells of the yeast Saccharomyces cerevisiae." Journal of Bacteriology 160(3): 1196-1198.
    Diploid formation by haploid cell of Saccharomyces cerevisiae was tested during and after treatment with chemical agents which bring about arrest at the cell cycle regulatory step 'start'. All compounds, except sinefungin, ...
  • Methionine analogs and cell division regulation in the yeast Saccharomyces cerevisiae 

    Singer, R. A., G. C. Johnston, and D. Bedard. 1978. "Methionine analogs and cell division regulation in the yeast Saccharomyces cerevisiae." Proceedings of the National Academy of Sciences of the United States of America 75(12): 6083-6087.
    No abstract available.
  • Nalidixic acid causes a transient G1 arrest in the yeast Saccharomyces cerevisiae 

    Singer, R. A., and G. C. Johnston. 1979. "Nalidixic acid causes a transient G1 arrest in the yeast Saccharomyces cerevisiae." Molecular and General Genetics 176(1): 37-39.
    No abstract available.
  • Activity of specific lipid-regulated ADP ribosylation factor-GTPase-activating proteins is required for Sec14p-dependent Golgi secretory function in yeast 

    Yanagisawa, L. L., J. Marchena, Z. Xie, X. Li, et al. 2002. "Activity of specific lipid-regulated ADP ribosylation factor-GTPase-activating proteins is required for Sec14p-dependent Golgi secretory function in yeast." Molecular biology of the cell 13(7): 2193-2206.
    Yeast phosphatidylinositol transfer protein (Sec14p) coordinates lipid metabolism with protein-trafficking events. This essential Sec14p requirement for Golgi function is bypassed by mutations in any one of seven genes ...
  • The yeast Arf GTPase-activating protein Age1 is regulated by phospholipase D for post-Golgi vesicular transport 

    Benjamin, J. J. R., P. P. Poon, S. M. Lewis, A. Auger, et al. 2011. "The yeast Arf GTPase-activating protein Age1 is regulated by phospholipase D for post-Golgi vesicular transport." Journal of Biological Chemistry 286(7): 5187-5196.
    Vesicular transport shuttles cargo among intracellular compartments. Several stages of vesicular transport are mediated by the small GTPase Arf, which is controlled in a cycle of GTP binding and hydrolysis by Arf ...
  • Dysregulated Arl1, a regulator of post-Golgi vesicle tethering, can inhibit endosomal transport and cell proliferation in yeast 

    Benjamin, J. J. R., P. P. Poon, J. D. Drysdale, X. Wang, et al. 2011. "Dysregulated Arl1, a regulator of post-Golgi vesicle tethering, can inhibit endosomal transport and cell proliferation in yeast." Molecular biology of the cell 22(13): 2337-2347.
    Small monomeric G proteins regulated in part by GTPase-activating proteins (GAPs) are molecular switches for several aspects of vesicular transport. The yeast Gcs1 protein is a dual-specificity GAP for ADP-ribosylation ...
  • Sug1 modulates yeast transcription activation by Cdc68 

    Xu, Q., R. A. Singer, and G. C. Johnston. 1995. "Sug1 modulates yeast transcription activation by Cdc68." Molecular and cellular biology 15(11): 6025-6035.
    The Cdc68 protein is required for the transcription of a variety of genes in the yeast Saccharomyces cerevisiae. In a search for proteins involved in the activity of the Cdc68 protein, we identified four suppressor genes ...
  • Transient cell cycle arrest of Saccharomyces cerevisiae by amino acid analog (beta)-2-DL-thienylalanine 

    Bedard, D. P., R. A. Singer, and G. C. Johnson. 1980. "Transient cell cycle arrest of Saccharomyces cerevisiae by amino acid analog (beta)-2-DL-thienylalanine." Journal of Bacteriology 141(1): 100-105.
    No abstract available.
  • FACT, the Bur kinase pathway, and the histone co-repressor HirC have overlapping nucleosome-related roles in yeast transcription elongation 

    Stevens, J. R., A. F. O'Donnell, T. E. Perry, J. J. R. Benjamin, et al. 2011. "FACT, the Bur kinase pathway, and the histone co-repressor HirC have overlapping nucleosome-related roles in yeast transcription elongation." PLoS ONE 6(10).
    Gene transcription is constrained by the nucleosomal nature of chromosomal DNA. This nucleosomal barrier is modulated by FACT, a conserved histone-binding heterodimer. FACT mediates transcription-linked nucleosome disassembly ...
  • Membrane metabolism mediated by Sec14 family members influences Arf GTPase activating protein activity for transport from the trans-Golgi 

    Wong, T. A., G. D. Fairn, P. P. Poon, M. Shmulevitz, et al. 2005. "Membrane metabolism mediated by Sec14 family members influences Arf GTPase activating protein activity for transport from the trans-Golgi." Proceedings of the National Academy of Sciences of the United States of America 102(36): 12777-12782.
    The budding yeast Saccharomyces cerevisiae contains a family of Arf (ADP-ribosylation factor) GTPase activating protein (GAP) proteins with the Gcs1 + Age2 ArfGAP pair providing essential overlapping function for the ...
  • Prenylated isoforms of yeast casein kinase I, including the novel Yck3p, suppress the gcs1 blockage of cell proliferation from stationary phase 

    Wang, X., M. F. Hoekstra, A. J. DeMaggio, N. Dhillon, et al. 1996. "Prenylated isoforms of yeast casein kinase I, including the novel Yck3p, suppress the gcs1 blockage of cell proliferation from stationary phase." Molecular and cellular biology 16(10): 5375-5385.
    The GCS1 gene of the budding yeast Saccharomyces cerevisiae mediates the resumption of cell proliferation from the starved, stationary-phase state. Here we identify yeast genes that, in increased dosages, overcome the ...
  • rRNA transcription initiation is decreased by inhibitors of the yeast cell cycle control step 'start' 

    Veinot-Drebot, L. M., R. A. Singer, and G. C. Johnston. 1989. "rRNA transcription initiation is decreased by inhibitors of the yeast cell cycle control step 'start'." Journal of Biological Chemistry 264(33): 19528-19534.
    Inhibitors of the 'start' regulatory step in the cell cycle of the yeast Saccharomyces cerevisiae are known by indirect studies to perturb RNA metabolism. We have investigated these effects further and show here by a ...
  • The Saccharomyces cerevisiae Cdc68 transcription activator is antagonized by San1, a protein implicated in transcriptional silencing 

    Xu, Q., G. C. Johnston, and R. A. Singer. 1993. "The Saccharomyces cerevisiae Cdc68 transcription activator is antagonized by San1, a protein implicated in transcriptional silencing." Molecular and cellular biology 13(12): 7553-7565.
    The CDC68 gene (also called SPT16) encodes a transcription factor for the expression of a diverse set of genes in the budding yeast Saccharomyces cerevisiae. To identify other proteins that are functionally related to the ...
  • Rapid initial cleavage of nascent pre-rRNA transcripts in yeast. 

    Veinot-Drebot, L. M., R. A. Singer, and G. C. Johnston. 1988. "Rapid initial cleavage of nascent pre-rRNA transcripts in yeast.." Journal of Molecular Biology 199(1): 107-113.
    In yeast cells, as in many other eukaryotes, the initial step in the processing of the pre-rRNA primary transcript is removal of external transcribed spacer (ETS) sequences from the 5' end of the transcript. We show here, ...
  • Saccharomyces cerevisiae Gcs1 is an ADP-ribosylation factor GTPase- activating protein 

    Pak Phi Poon, , X. Wang, M. Rotman, I. Huber, et al. 1996. "Saccharomyces cerevisiae Gcs1 is an ADP-ribosylation factor GTPase- activating protein." Proceedings of the National Academy of Sciences of the United States of America 93(19): 10074-10077.
    Movement of material between intracellular compartments takes place through the production of transport vesicles derived from donor membranes. Vesicle budding that results from the interaction of cytoplasmic coat proteins ...
  • The Gcs1 Arf-GAP mediates Snc1,2 v-SNAKE retrieval to the Golgi in yeast 

    Robinson, M., P. P. Poon, C. Schindler, L. E. Murray, et al. 2006. "The Gcs1 Arf-GAP mediates Snc1,2 v-SNAKE retrieval to the Golgi in yeast." Molecular biology of the cell 17(4): 1845-1858.
    Gcs1 is an Arf GTPase-activating protein (Arf-GAP) that mediates Golgi-ER and post-Golgi vesicle transport in yeast. Here we show that the Snc1,2 v-SNAREs, which mediate endocytosis and exocytosis, interact physically and ...
  • Retrograde transport from the yeast Golgi is mediated by two ARF GAP proteins with overlapping function 

    Poon, P. P., D. Cassel, A. Spang, M. Rotman, et al. 1999. "Retrograde transport from the yeast Golgi is mediated by two ARF GAP proteins with overlapping function." EMBO Journal 18(3): 555-564.
    ARF proteins, which mediate vesicular transport, have little or no intrinsic GTPase activity. They rely on the actions of GTPase-activating proteins (GAPs) for their function. The in vitro GTPase activity of the Saccharomyces ...

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