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IDENTIFICATION AND CHARACTERIZATION OF PROMYELOCYTIC LEUKEMIA (PML)-ISOFORM 1 SPECIFIC PROTEIN-PROTEIN INTERACTIONS

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dc.contributor.author Tse, Brenda
dc.date.accessioned 2012-09-11T11:40:14Z
dc.date.available 2012-09-11T11:40:14Z
dc.date.issued 2012-09-11
dc.identifier.uri http://hdl.handle.net/10222/15493
dc.description.abstract Loss of the promyelocytic leukemia (PML) protein is associated with genomic instability/cancer. There are several isoforms of the PML protein that localize in PML nuclear bodies (PML NBs). How each individual isoform contributes to the functions of PML NBs is unknown. The objective of this study was to identify and characterize PML isoform-I (PML-I) specific protein-protein interactions. Using yeast two-hybrid screens, several interacting partners of PML-I were identified that play roles in translational regulation, including eukaryotic initiation factor 3 subunit K (eIF3K). Our studies demonstrated that eIF3K interacts with PML-I in vitro and in vivo. Through its interaction with eIF3K, overexpression of PML-I resulted in the concomitant increase in eIF3K protein levels in mammalian cells. This suggests that PML-I may be involved in regulating eIF3K protein translation or stability, which in turn could affect translation of specific mRNAs or global translation in cancer cells with reduced expression of PML-I. en_US
dc.language.iso en en_US
dc.subject PML and PML isoforms en_US
dc.subject PML nuclear bodies en_US
dc.subject eIF3K en_US
dc.subject Translation initiation en_US
dc.subject Yeast two-hybrid screen en_US
dc.title IDENTIFICATION AND CHARACTERIZATION OF PROMYELOCYTIC LEUKEMIA (PML)-ISOFORM 1 SPECIFIC PROTEIN-PROTEIN INTERACTIONS en_US
dc.date.defence 2011-04-18
dc.contributor.department Department of Pathology en_US
dc.contributor.degree Master of Science en_US
dc.contributor.external-examiner Dr. Catherine Too en_US
dc.contributor.graduate-coordinator Dr. Wenda Greer en_US
dc.contributor.thesis-reader Dr. Melanie Dobson en_US
dc.contributor.thesis-reader Dr. David Waisman en_US
dc.contributor.thesis-supervisor Dr. Graham Dellaire en_US
dc.contributor.ethics-approval Not Applicable en_US
dc.contributor.manuscripts Not Applicable en_US
dc.contributor.copyright-release Not Applicable en_US


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