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PURIFICATION OF CHYMOTRYPSIN FROM FISH WASTE USING REVERSE MICELLES

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dc.contributor.author Zhou, Liang
dc.date.accessioned 2011-12-14T18:34:13Z
dc.date.available 2011-12-14T18:34:13Z
dc.date.issued 2011-12-14
dc.identifier.uri http://hdl.handle.net/10222/14353
dc.description Fish processing waste can be used to produce commercially valuable by-products, such as chymotrypsin which has application in various industries including the food industry, leather production industry and chemical industry. My project is to produce valuable by-products from the fish processing. en_US
dc.description.abstract Reverse micelles systems AOT/isooctane was used for the concentration chymotrypsin from crude aqueous extract of red perch (intestine). The effects of pH and AOT concentration in the forward extraction step and pH and KCl concentration in the backward extraction step on the enzyme activity, purification fold and recovery yield were studied. The optimum conditions for the forward extraction were AOT concentration 20 mM and pH of 7.0 and optimum conditions for backward extraction were KCl concentration 1.0 M and pH of 7.5 which gave a good recovery yield (102.24%) and a purification (32.24-fold). The addition of 15% v/v alcohol in backward extraction dramatically improved recovery yield by 4.5 times and purification by 2.5 times. The enzyme activity and recovery yield obtained using reverse micelles method under its optimal conditions were 2 fold higher than those obtained using the ammonium sulphate precipitation method, while purification fold were 3 fold higher. en_US
dc.language.iso en en_US
dc.subject Chymotrypsin en_US
dc.subject fish waste en_US
dc.subject extraction en_US
dc.subject purification en_US
dc.subject enzyme activity en_US
dc.subject microemulsions en_US
dc.subject fractionation en_US
dc.title PURIFICATION OF CHYMOTRYPSIN FROM FISH WASTE USING REVERSE MICELLES en_US
dc.date.defence 2011-11-23
dc.contributor.department Department of Process Engineering and Applied Science en_US
dc.contributor.degree Master of Science en_US
dc.contributor.external-examiner Abdel E. Ghaly en_US
dc.contributor.graduate-coordinator George Kipouros en_US
dc.contributor.thesis-reader Abdel E. Ghaly en_US
dc.contributor.thesis-reader Suzanne M. Budge en_US
dc.contributor.thesis-reader Su-Ling Brooks en_US
dc.contributor.thesis-supervisor Abdel E. Ghaly, Suzanne M. Budge en_US
dc.contributor.ethics-approval Not Applicable en_US
dc.contributor.manuscripts Not Applicable en_US
dc.contributor.copyright-release Not Applicable en_US


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