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dc.contributor.authorZhou, Liang
dc.date.accessioned2011-12-14T18:34:13Z
dc.date.available2011-12-14T18:34:13Z
dc.date.issued2011-12-14
dc.identifier.urihttp://hdl.handle.net/10222/14353
dc.descriptionFish processing waste can be used to produce commercially valuable by-products, such as chymotrypsin which has application in various industries including the food industry, leather production industry and chemical industry. My project is to produce valuable by-products from the fish processing.en_US
dc.description.abstractReverse micelles systems AOT/isooctane was used for the concentration chymotrypsin from crude aqueous extract of red perch (intestine). The effects of pH and AOT concentration in the forward extraction step and pH and KCl concentration in the backward extraction step on the enzyme activity, purification fold and recovery yield were studied. The optimum conditions for the forward extraction were AOT concentration 20 mM and pH of 7.0 and optimum conditions for backward extraction were KCl concentration 1.0 M and pH of 7.5 which gave a good recovery yield (102.24%) and a purification (32.24-fold). The addition of 15% v/v alcohol in backward extraction dramatically improved recovery yield by 4.5 times and purification by 2.5 times. The enzyme activity and recovery yield obtained using reverse micelles method under its optimal conditions were 2 fold higher than those obtained using the ammonium sulphate precipitation method, while purification fold were 3 fold higher.en_US
dc.language.isoenen_US
dc.subjectChymotrypsinen_US
dc.subjectfish wasteen_US
dc.subjectextractionen_US
dc.subjectpurificationen_US
dc.subjectenzyme activityen_US
dc.subjectmicroemulsionsen_US
dc.subjectfractionationen_US
dc.titlePURIFICATION OF CHYMOTRYPSIN FROM FISH WASTE USING REVERSE MICELLESen_US
dc.date.defence2011-11-23
dc.contributor.departmentDepartment of Process Engineering and Applied Scienceen_US
dc.contributor.degreeMaster of Scienceen_US
dc.contributor.external-examinerAbdel E. Ghalyen_US
dc.contributor.graduate-coordinatorGeorge Kipourosen_US
dc.contributor.thesis-readerAbdel E. Ghalyen_US
dc.contributor.thesis-readerSuzanne M. Budgeen_US
dc.contributor.thesis-readerSu-Ling Brooksen_US
dc.contributor.thesis-supervisorAbdel E. Ghaly, Suzanne M. Budgeen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseNot Applicableen_US
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