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Potential roles of the chaperonin (HtpB), polyamines, and the polyamine binding protein (PotD) in Legionella pneumophila pathogenesis

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dc.contributor.author Nasrallah, Gheyath K.
dc.date.accessioned 2011-09-06T14:05:39Z
dc.date.available 2011-09-06T14:05:39Z
dc.date.issued 2011-09-06
dc.identifier.uri http://hdl.handle.net/10222/14200
dc.description.abstract The intracellular pathogen Legionella pneumophila replicates in a membrane-bound compartment known as the Legionella-containing vacuole (LCV) where it abundantly releases its chaperonin HtpB, suggesting that HtpB may have virulence-related functions. To assess these functions, I attempted to construct an L. pneumophila ?htpBmutant but was unable to do so, likely because htpB is essential. In the absence of genetic deletion, functional tests were used to study the released HtpB. A small portion of the HtpB in L. pneumophila-infected cells was found in the cytoplasm of the infected cells, as judged by the CyaA reporter assay. To identify potential functions of the HtpB present in the eukaryotic cytoplasm, htpB was ectopically expressed in Saccharomyces cerevisiae. HtpB induced pseudohyphal growth (PHG) in yeast, suggesting it interacts with eukaryotic targets. A yeast two-hybrid screen showed that HtpB interacted with SAMDC, an essential yeast enzyme encoded by SPE2 that is required for polyamine biosynthesis. Overexpression of SPE2 induced PHG in S. cerevisiae, suggesting that HtpB induces PHG by activating polyamine synthesis, and that L. pneumophila may require exogenous polyamines for growth. A pharmacological inhibitor of SAMDC reduced L. pneumophila replication in host cells, whereas exogenous polyamines enhanced intracellular growth. Bioinformatics revealed that most known enzymes required for polyamine biosynthesis in bacteria are absent in L. pneumophila, suggesting that L. pneumophila depends on exogenous polyamines transported from host cells. L. pneumophila possesses only one putative operon,potABCD, which encodes a polyamine transporter. Using GFP as a reporter of potABCD promoter (PpotA), we found that PpotA activity was turned on during exponential phase of growth in vitro. To test the potential function of this transporter in pathogenesis, potD was deleted.Although deletion of potD did not affect L. pneumophila growth in vitro, it reduced L. pneumophila attachment to phagocytic cells, intracellular growth, and the ability of the LCV to recruit vesicles. Collectively, these findings have contributed to a better understanding of the biology of L. pneumophila by suggesting that HtpB and PotD might collaborate to ensure a supply of polyamines required for the optimal intracellular growth of L. pneumophila. en_US
dc.language.iso en en_US
dc.subject Legionella, chaperonin, polyamines, intracellular growth en_US
dc.title Potential roles of the chaperonin (HtpB), polyamines, and the polyamine binding protein (PotD) in Legionella pneumophila pathogenesis en_US
dc.date.defence 2011-05-17
dc.contributor.department Department of Microbiology & Immunology en_US
dc.contributor.degree Doctor of Philosophy en_US
dc.contributor.external-examiner Carmen Buchrieser en_US
dc.contributor.graduate-coordinator Dr. Roy Duncan en_US
dc.contributor.thesis-reader Dr. Jessica Boyd en_US
dc.contributor.thesis-reader Dr. Ross Davidson en_US
dc.contributor.thesis-reader Dr. Chris Richardson en_US
dc.contributor.thesis-supervisor Dr. Rafael Garduno and Dr. Lois Murray en_US
dc.contributor.ethics-approval Not Applicable en_US
dc.contributor.manuscripts Yes en_US
dc.contributor.copyright-release Yes en_US

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