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The Role of K+ Channels in Cell Proliferation and Cell Volume Regulation in Human Bronchial Epithelial Cells

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dc.contributor.author MacLeod, Katie
dc.date.accessioned 2011-08-19T11:43:48Z
dc.date.available 2011-08-19T11:43:48Z
dc.date.issued 2011-08-19
dc.identifier.uri http://hdl.handle.net/10222/14077
dc.description.abstract K+ channels are expressed in a wide variety of cell types, including airway epithelial cells. The purpose of this study was to investigate the role of various Kv and KCa channels in cell proliferation and cell volume regulation in HBE and CFBE cell lines. Methods included mRNA and protein detection, cell counting and cytotoxicity assays, and cell volume experiments. HBE and CFBE cells expressed many K+ channel mRNA and protein, including Kv7.1, Kv7.5, Kv4.1, Kv4.2, Kv10.1, Kv11.1, KCa1.1 and KCa3.1. Various Kv and KCa channels played a role in HBE and CFBE basal cell proliferation, as well as in mediating the regulatory volume decrease (RVD) response in HBE cells. CFBE cells did not elicit an RVD response and neither cell line underwent a regulatory volume increase (RVI) response. Therefore, K+ channels play an important role in cell proliferation and the regulation of cell volume in bronchial epithelial physiology. en_US
dc.language.iso en en_US
dc.title The Role of K+ Channels in Cell Proliferation and Cell Volume Regulation in Human Bronchial Epithelial Cells en_US
dc.date.defence 2011-08-05
dc.contributor.department Department of Physiology & Biophysics en_US
dc.contributor.degree Master of Science en_US
dc.contributor.external-examiner Dr. Boris Kablar en_US
dc.contributor.external-examiner Dr. Jonathan Blay en_US
dc.contributor.graduate-coordinator Dr. Paul Murphy en_US
dc.contributor.thesis-reader Dr. Robert Rose en_US
dc.contributor.thesis-reader Dr. Nikolas Morgunov en_US
dc.contributor.thesis-supervisor Dr. Elizabeth Cowley en_US
dc.contributor.ethics-approval Not Applicable en_US
dc.contributor.manuscripts Not Applicable en_US
dc.contributor.copyright-release Not Applicable en_US


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