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THE EFFECT OF INSULIN ON STRESS-RESPONSE PATHWAYS IN A CELLULAR MODEL OF RAT CARDIOMYOCYTES

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dc.contributor.author Jones, Quinton RD
dc.date.accessioned 2011-08-15T17:30:48Z
dc.date.available 2011-08-15T17:30:48Z
dc.date.issued 2011-08-15
dc.identifier.uri http://hdl.handle.net/10222/14058
dc.description Insulin was used on a model on H9c2 myotubes to determine the effect of oxygen-glucose deprivation and reoxygenation on the localization and phosphorylation of Hsp27 and p38 MAPK en_US
dc.description.abstract Insulin and cellular stressors both activate p38 MAPK. Insulin protects cardiac tissue in a p38 MAPK-dependent manner. Paradoxically, inhibiting p38 MAPK is also protective. Hsp27 phosphorylation is regulated by p38 MAPK. Insulin was tested in H9c2 cardiomyocytes subjected to media exchange, 6 hours of oxygen-glucose deprivation, and reoxygenation. Insulin suppressed stress-induced phosphorylation of Hsp27 due to media-exchange or oxygen-glucose deprivation. Surprisingly, insulin increased Hsp27 phosphorylation during reoxygenation. Insulin also reduced total p38 MAPK levels. Insulin before oxygen-glucose deprivation prevented both localization of Hsp27 to the nucleus and localization of phospho-p38 MAPK to the cytoplasm. Insulin during oxygen-glucose deprivation caused the localization of phospho-p38 MAPK in the cytoplasm, but did not increase Hsp27 phosphorylation until reoxygenation. In conclusion, insulin may protect before oxygen-glucose deprivation by redirecting phospho-p38 MAPK to the nucleus away from damaging pathways in the cytoplasm and protects during oxygen-glucose deprivation by priming phospho-p38 MAPK to phosphorylate Hsp27. en_US
dc.language.iso en en_US
dc.subject insulin h9c2 cardiomyocytes OGD anoxia ischemia hsp27 p38 mapk en_US
dc.title THE EFFECT OF INSULIN ON STRESS-RESPONSE PATHWAYS IN A CELLULAR MODEL OF RAT CARDIOMYOCYTES en_US
dc.date.defence 2011-08-05
dc.contributor.department Department of Anatomy & Neurobiolgy with Neuroscience en_US
dc.contributor.degree Master of Science en_US
dc.contributor.external-examiner Bill Baldridge en_US
dc.contributor.graduate-coordinator Kazue Semba en_US
dc.contributor.thesis-reader William Currie, Kazue Semba, Gary Allen, Bill Baldridge, Eileen Denovan-Wright en_US
dc.contributor.thesis-supervisor William Currie en_US
dc.contributor.ethics-approval Not Applicable en_US
dc.contributor.manuscripts Not Applicable en_US
dc.contributor.copyright-release Not Applicable en_US


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