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dc.contributor.authorMacIntosh, Andrew John
dc.date.accessioned2010-09-09T13:52:32Z
dc.date.available2010-09-09T13:52:32Z
dc.date.issued2010-09-09
dc.identifier.urihttp://hdl.handle.net/10222/13046
dc.description.abstractThe bacteria Streptomyces venezuelae produce the novel antibiotic jadomycin. The study of growth characteristics and metabolic behavior of the bacteria are necessary to scale up antibiotic production and facilitate further research. In this study, a method for producing consistent inoculum was developed that showed good repeatability when used in growth trials. The rod shaped spores of Streptomyces venezuelae were determined to be approximately 0.8 x 0.2 ?m with a smooth surface type. The effects of temperature and pH on bacterial growth and substrate consumption were examined in a 7 L bioreactor. Of the range of parameters tested (28, 32, 36 °C, and media pH of 5, 7 and 9), 32 °C with a media pH of 7 yielded the highest rate of growth (µmax of 1.43 hours-1 with a lag time of 7.7 hours). The results of all trials showed that free glucose was consumed before the maltose, which was the major sugar substrate in the media. The initiation of exponential bacterial growth occurred after rapid consumption of free glucose. A heat balance analysis was also performed over the bioreactor to identify the heat generated through agitation, losses over the vessel and the heat of metabolism from Streptomyces venezuelae. Under normal operating parameters 33 - 24 % of the heat generated through mixing was lost with the exhaust gas, while 56 - 64 % was lost through the bioreactor wall. The heat of mixing was calculated to be 1.62 J•s-1 while the maximum amount of heat generated by Streptomyces venezuelae metabolism and activity during a growth trial was 2.28 J•s-1 for 60 x 109 CFU?mL-1.en_US
dc.language.isoenen_US
dc.subjectjadomycinen_US
dc.subjectheat balanceen_US
dc.subjectgrowth rateen_US
dc.subjectsubstrate consumptionen_US
dc.subjectStreptomyces venezuelaeen_US
dc.subjectspore inoculumen_US
dc.titleA STUDY ON THE GROWTH AND METABOLIC ACTIVITY OF STREPTOMYCES VENEZUELAEen_US
dc.date.defence2010-08-19
dc.contributor.departmentDepartment of Biological Engineeringen_US
dc.contributor.degreeMaster of Applied Scienceen_US
dc.contributor.external-examinerDr. M. Walshen_US
dc.contributor.graduate-coordinatorDr. G. Kipourosen_US
dc.contributor.thesis-readerDr. P. Gratzeren_US
dc.contributor.thesis-supervisorDr.Su-Ling. Brooksen_US
dc.contributor.ethics-approvalNot Applicableen_US
dc.contributor.manuscriptsNot Applicableen_US
dc.contributor.copyright-releaseNot Applicableen_US
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