dc.contributor.author | Balasuriya, Nileeka | |
dc.date.accessioned | 2011-12-19T16:32:38Z | |
dc.date.available | 2011-12-19T16:32:38Z | |
dc.date.issued | 2011-12-19 | |
dc.identifier.uri | http://hdl.handle.net/10222/14389 | |
dc.description.abstract | Hypertension is a major public health problem rising across the globe. Inhibition of angiotensin converting enzyme (ACE) is identified as a main therapeutic target in controlling high blood pressure. The current research study investigated the ACE inhibitory property of a flavonoid-rich apple peel extract (FAE), its constituents, selected flavonoids and some quercetin metabolites using a biochemical assay of ACE inhibition and a human umbilical vein endothelial cell (HUVEC) model. FAE, all the tested flavonoids except genistein, and two quercetin metabolites (quercetin-3-O-glucuronic acid and quercetin-3-O-sulfate) significantly (p < 0.05) inhibited ACE. Enzyme kinetic analysis proved that flavonoids are competitive inhibitors over ACE. In the HUVEC model FAE, quercetin-3-O-glucoside and quercetin-3-O-glucuronic acid significantly (p < 0.05) inhibited ACE. Overall, FAE and most of the flavonoids tested showed ACE inhibition in vitro which needs further investigations using animal and human clinical trials. | en_US |
dc.language.iso | en | en_US |
dc.title | Antihypertensive Properties of Flavonoid-Rich Apple Peel Extract, Selected Flavonoids and Metabolites In Vitro | en_US |
dc.date.defence | 2011-12-12 | |
dc.contributor.department | Nova Scotia Agricultural College | en_US |
dc.contributor.degree | Master of Science | en_US |
dc.contributor.external-examiner | Dr.Katherine Gottschall-Pass | en_US |
dc.contributor.graduate-coordinator | Dr.Dian Patterson | en_US |
dc.contributor.thesis-reader | Dr.Robin Robinson, Dr.David Barrett | en_US |
dc.contributor.thesis-supervisor | Dr.Vasantha Rupasignhe | en_US |
dc.contributor.ethics-approval | Not Applicable | en_US |
dc.contributor.manuscripts | Not Applicable | en_US |
dc.contributor.copyright-release | Not Applicable | en_US |