| dc.contributor.author | You, YongDong. | en_US |
| dc.date.accessioned | 2014-10-21T12:33:40Z | |
| dc.date.available | 1993 | |
| dc.date.issued | 1993 | en_US |
| dc.identifier.other | AAINN93760 | en_US |
| dc.identifier.uri | http://hdl.handle.net/10222/55402 | |
| dc.description | This study investigated the electrophysiological and morphological alterations in renal proximal tubules following exposure to ischemic conditions and preservation solutions, using an in vitro single-tubule perfusion model. | en_US |
| dc.description | Isolated mouse proximal straight tubules were subjected to simulated ischemic conditions by perfusion with hypoxic and ischemic solutions (Po$\sb2$ 4 mmHg); the latter solution was high in K$\sp+$ and lactate, devoid of glucose, and low in pH. Twenty-minute tubular perfusion with the hypoxic or ischemic solution on both the luminal and the basolateral sides at $37\sp\circ\rm C$ did not significantly alter cell membrane potential, cell membrane ionic conductance, and intracellular Na$\sp+$ activity. The ultrastructure of the tubular cells was also well preserved following the perfusion. However, such a perfusion decreased both the transepithelial potential and resistance by 40%. These results suggest that tubular cells are able to maintain their structural and electrochemical integrity after short-term exposure to hypoxic or ischemic conditions. The compromised transepithelial transport would seem to indicate that cell-to-cell junctions are damaged by these insults. | en_US |
| dc.description | Proximal tubules were also perfused with Euro-Collins' (EC) and University of Wisconsin (UW) solutions at 22$\sp\circ$C and 37$\sp\circ$C. Perfusion with the EC solution on both the luminal and the basolateral sides led to significant cell swelling, accompanied by deterioration of the transepithelial potential upon reperfusion with the Ringer solution. When the EC solution was perfused on the luminal and the basolateral sides separately, it was found that the deteriorating effect of this solution is associated with its presence on the basolateral side. In comparison, perfusion with the UW solution had no effect on cell volume, and no subsequent damage to the transepithelial potential was observed. Furthermore, substitution of glucose with mannitol abolished the damaging effect of the EC solution. These data emphasize the importance of effective membrane impermeants in the preservation solutions in maintaining cell volume, and suggest that cell swelling in the EC solution at warm temperatures could lead to acute tubular necrosis and delayed graft function in clinical renal transplantation. | en_US |
| dc.description | Thesis (Ph.D.)--Dalhousie University (Canada), 1993. | en_US |
| dc.language | eng | en_US |
| dc.publisher | Dalhousie University | en_US |
| dc.publisher | | en_US |
| dc.subject | Biology, Animal Physiology. | en_US |
| dc.title | Effects of ischemia and preservation solutions on morphology and transport of renal proximal tubules. | en_US |
| dc.type | text | en_US |
| dc.contributor.degree | Ph.D. | en_US |
