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dc.contributor.authorKamonrat, Wongpathom.en_US
dc.date.accessioned2014-10-21T12:38:16Z
dc.date.available1996
dc.date.issued1996en_US
dc.identifier.otherAAINN16023en_US
dc.identifier.urihttp://hdl.handle.net/10222/55157
dc.descriptionMicrosatellite DNA markers were developed from a Thai silver barb (Puntius gonionotus) genomic library and were then used to study various aspects of the genetics of P. gonionotus populations in Thailand. The aim is to provide means for evaluating management policies for the species in terms of conservation and genetic improvement.en_US
dc.descriptionTwelve natural populations from three rivers and 29 hatchery stocks from the central and north-east regions of Thailand were studied. Genetic variability was high in both groups of populations. Multidimensional scaling analysis of genetic distances revealed the discreteness apparent between watersheds among natural populations, and between geographic regions among hatchery stocks. High genetic variability within populations and significant genetic differentiation between populations both in native and hatcheries stocks indicate rich genetic resources of this species in Thailand. However, there was evidences that stock management may pose a threat of losing or altering genetic integrity of both natural and hatchery populations. Mixed stock analysis of the fish sampled from the rivers indicated 75% to 96% were from hatchery populations. This high genetic contamination of the natural populations was undoubtedly the consequence of restocking programs in which millions of P. gonionotus are released to rivers each year. Evidence of reduction of genetic integrity between regions was also observed in stations due to stock transfer. The results suggested an urgent need for genetically based stock management policies for both natural and hatchery populations.en_US
dc.descriptionThe potential use of microsatellites for broodstock improvement in aquaculture was studied. Pedigrees of individuals were successfully established in a large communal rearing by using one to five microsatellites. The ability to identify individuals allowed a complicated genetic experiment and selective breeding to be conducted in places where facilities were limited. Results are considered to be more reliable because environmental variances are accounted for as fish are grown together from birth. In this study, heritability of growth traits in three stocks of P. gonionotus were estimated where all families were reared together. The estimates ranged from 0.193 to 0.523 suggesting that selective breeding in this species should result in good progress. However, heterozygosity in the largest individuals was greatly reduced, indicating that rapid inbreeding is very likely in simple means selection strategies.en_US
dc.descriptionThesis (Ph.D.)--Dalhousie University (Canada), 1996.en_US
dc.languageengen_US
dc.publisherDalhousie Universityen_US
dc.publisheren_US
dc.subjectBiology, Genetics.en_US
dc.subjectAgriculture, Fisheries and Aquaculture.en_US
dc.titleSpatial genetic structure of Thai silver barb Puntius gonionotus (Bleeker) populations in Thailand.en_US
dc.typetexten_US
dc.contributor.degreePh.D.en_US
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